NextGENe software also has an enhanced processing optimization providing 60% faster processing time. Information from these database can be used for display and/or filtering the Mutation Report. Additionally, the NextGENe Track Manager Tool enables users to quickly import the most recent data from dbNSFP or custom databases. Updated dbSNP information is included in the human genome reference provided by SoftGenetics as well as select GenBank files downloaded from NCBI, providing hyperlinked access from the mutation report. Reports also include a host of filtering options, variant confidence scoring, multiple analysis comparison capabilities, hyperlinks to dbSNP, and additional tracks with causative prediction through dbNSFP, as well as custom database import including ExAC, gnomAD, and NHLBI GO Exome Sequencing Project (ESP).įigure 2: Mutation report with custom track information, including 1000 Genomes and dbNSFP.ĭatabase information can easily be imported in NextGENe software. Upon completion of a sequence alignment project, interactive mutation reports are automatically generated providing detailed variant and annotation information. Alignment and variant detection can be completed using a whole genome reference or targeted regions using GenBank or fasta files. NextGENe software features up to 99% accuracy, with the ability to detect indels up to the read length, and rapid review of DNA variants, nucleotides, and amino acids. In addition to powerful analysis tools, NextGENe software offers automated and advanced creation of custom reports and quality metrics as well as a biologist friendly annotated whole genome browser/viewer for review, filtering, editing, and commenting.įigure 1: NextGENe Viewer software displaying a SNP with purple background. NextGENe software combines patented algorithms and analysis tools designed to run on a Windows® operating system, eliminating the need for scripting and other bioinformatics support needed in programs such as CLC Genomics Workbench, Lasergene's SeqMan Pro, as well as academic software such as MAQ & SOAP, Top Hat, BWA & Bowtie. NextGENe® software has been developed specifically for use by biologists performing whole genome sequencing (WGS), whole exome sequencing (WES), or targeted resequencing projects containing single nucleotide polymorphisms (SNPs), Insertions/deletions (indels), and large structural DNA rearrangements from Next Generation Sequencing data produced by Illumina® iSeq, Miniseq, MiSeq, NextSeq, HiSeq, and NovaSeq systems, Ion Torrent Ion GeneStudio S5, PGM, and Proton systems as well as other platforms. Next Generation Sequencing has made it possible to rapidly examine individual genomes and compare genetic sequences across multiple genomes to detect variants. Also added a non-linearity to zooming (starts slower).SNP/Indel Detection of Next Generation Sequence (NGS) Reads I.e., the bigger the tree the more you can zoom in. The maximum extent of zooming is now proportional to the number of tips in the tree. These include the reintroduction of font size spinners (in addition to font selection dialogs) and scrolling of the entire control panel when larger than the window. Numerous tweaks to the controls to make things more convenient.Colour schemes are now selectable for particular attributes and these are saved and loaded in FigTree files.The old interpolating colour scheme is still available. New colour scheme for continuous attributes also based on HSB.This replaces the old fixed sequence of colours and is more customizable. New colour scheme for discrete attributes based on a HSB colour wheel.Added a 'Legend' option which can produce a colour legend for any attribute/colour scheme being used.These can be coloured and sized by attributes. Added a 'Node Shape' option which can draw circles, squares or diamonds on each node.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |